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300 mM NaOH, 1.0 mM Na2EDTA.
2,5 M NaCl, 100 mM Na2EDTA, 10 mM TRIS, 0,97% Sarkosyl, 1% Triton X-100, pH = 10.0.
C.I. 50040 0.4% = Neutral red. Synonyms: Toluylene red. Not identical with: C.I. 60760 = Nuclear fast red, Kernechtrot.
TRIS buffer solution pH = 7,50.
10-fold concentrated electrophoresis running buffer.0.4 M TRIS, 0.2 M acetic acid, 10 mM EDTA-Na2, pH = 8.5 ± 0.2, microfiltrated.TAE Buffer for electrophoresis of nucleic acids with lower buffer capacity than TBE. Linear dsDNA running in TAE faster than in TBE-Buffer. Best results with supercoiled DNA and compared with TBE better resolution of fragments > 4 kb.
10-fold concentrated electrophoresis running buffer.0.4 M TRIS, 0.2 M acetic acid, 1 mM EDTA-Na2, pH = 8.5 ± 0.2, microfiltrated.TAE buffer for electrophoresis with reduced EDTA-content.
10-fold concentrated electrophoresis running buffer.0.89 M TRIS, 0.89 M boric acid, 20 mM EDTA-Na2, pH = 8.3 ± 0.2, microfiltrated.TBE buffer for electrophoresis of nucleic acids with higher buffer capacity than TAE. Usable for DNA- and RNA-polyacrylamide- and agarose gel electrophoresis. TBE-Buffer is especially suited for electrophoresis for > 150 V and product with 0,1 ... 3,0 kb-fragments a better separation than TAE.